Fig. 4

Propidium iodide uptake of organotypic slices control (CTL) and with spontaneous epileptiform activity (EL). a Representative fluorescence photomicrographs of PI uptake in CTL and EL slices. b Quantification of PI uptake by each hippocampal region, expressed in arbitrary units of fluorescence intensity, was performed with ImageJ software. In both conditions, the highest PI uptake was observed in CA1 pyramidal region. However, EL slices have a higher PI incorporation in all hippocampal areas when compared to CTL ones. All values are mean ± SEM. n = 17–29 slices per condition, from 3 to 4 independent cultures. Statistical tests were performed with one-way ANOVA, followed by Bonferroni’s comparison test, *p < 0.05, for comparisons within the same hippocampal area, ^#p < 0.05, for comparisons within the same group, as depicted by the lines above the bars. Scale bar, 200 μm