Ex vivo model of epilepsy in organotypic slices—a new tool for drug screening

Table 1 Primers used for the analysis of proinflammatory cytokines by RT-PCR. All primers were designed using the OligoAnalyzer 3.1, provided by Integrated DNA Technologies

Gene	Acession number	Primer sequence (5′–3′)	PCR fragment size (bp)
Proinflammatory cytokines
IL-1β	NM_031512.2	Forward: TCCTCTGTGACTCGTGGGAT	309
IL-1β	NM_031512.2	Reverse: GTTTGGGATCCACACTCTCCA	309
TNF-α	NM_012675.3	Forward: ATGGGCTCCCTCTCATCAGT	106
TNF-α	NM_012675.3	Reverse: GCTTGGTGGTTTGCTACGAC	106
IL-6	NM_012589.2	Forward: GCAAGAGACTTCCAGCCAGT	203
IL-6	NM_012589.2	Reverse: TTGCCATTGCACAACTCTTTTCT	203
Housekeeping gene
GAPDH	NM_017008	Forward: GTTTGTGATGGGTGTGAACC	170
GAPDH	NM_017008	Reverse: TCTTCTGAGTGGCAGTGATG	170

The table indicates the gene, the gene accession number, the primer sequence, and the PCR fragment size

ISSN: 1742-2094