Fig. 2

CORM-3 treatment decreased the number of activated microglia at 3 days after ischemia. a Representative immunofluorescence staining of Iba-1-positive cells in each group. Insets show colocalization of the nuclear stain DAPI (blue). Yellow arrowheads point out activated microglia in peri-infarction zones. Scale bar = 50 μm. b Representative images show the morphology of resting (left) and activated (right) microglia. Scale bar = 10 μm. c Quantification indicates that CORM-3 significantly decreased the number of activated microglia (*p < 0.05, n = 8 per group). d Representative Western blot of Iba-1, TNF-α, and IL1β expression. e–g Quantification of the Western blot analysis (*p < 0.05 vs. Sham + saline group, ^#p < 0.05 vs. tMCAO + CORM-3 group, n = 10 per group). h, i ELISA analysis of TNF-α and IL1β (*p < 0.05 vs. Sham + saline group, ^#p < 0.05 vs. tMCAO + CORM-3 group, n = 8 per group). Data are shown as mean ± SD