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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Teriflunomide attenuates neuroinflammation-related neural damage in mice carrying human PLP1 mutations

Fig. 3

Therapeutic treatment fosters proliferation of regulatory CD8+ PD-1+ T-lymphocytes in optic nerves of PLPmut mice. a Representative example of the close apposition of a presumed effector T cell (CD8+ PD-1−, arrow) with a regulatory CD8+ PD-1+ T-lymphocyte (arrowhead) in the optic nerve of a 15-month-old therapeutically treated PLPmut mouse. b Quantification of immunocytochemically labeled CD8+ T cells in optic nerves of 15-month-old PLPmut mice revealed a relative increase of CD8+ PD-1+ regulatory T cells upon therapeutic treatment. c, d Quantitative triple-immunocytochemistry combining antibodies against CD8 (red), against PD-1 (green) and against the proliferation marker Ki67 (gray scale) revealed increased proliferation activity in regulatory CD8+ PD-1+ T-lymphocytes, but not in CD8+ PD-1− presumed effector T cells in optic nerves of therapeutically treated 15-month-old PLPmut mice. Scale bars: 10 μm. Kruskal-Wallis test and Bonferroni-Holm correction. *P < 0.05. n = 5 mice per group

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