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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Astrocytic gap junction inhibition by carbenoxolone enhances the protective effects of ischemic preconditioning following cerebral ischemia

Fig. 5

Ischemic preconditioning attenuated levels of functional Cx43 gap junction hemichannels and glutaminase mRNA expression in astrocytes. a Real-time quantitative PCR results. The relative expression of glutaminase (Gls) mRNA in all samples was quantified in comparison with glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Oxygen-glucose deprivation/re-oxygenation (OGD/R) increased Gls mRNA expression in astrocytes, but ischemic preconditioning (IP) and IP + carbenoxolone (CBX) reduced the expression of Gls mRNA. b Fluorescence microscopy shows that both IP alone and IP + CBX attenuate the function of Cx43 hemichannels at the cell surface of astrocytes. All scale bars represent 200 μm. c Quantification of the function of Cx43 hemichannels. NT, non-treated

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Journal of Neuroinflammation

ISSN: 1742-2094