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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Unveiling anti-oxidative and anti-inflammatory effects of docosahexaenoic acid and its lipid peroxidation product on lipopolysaccharide-stimulated BV-2 microglial cells

Fig. 1

ac Effects of DHA, 4-HHE, and 4-HNE on viability of BV-2 microglial cells. BV-2 microglial cells were cultured in 96-well plates until reaching 90% confluence. Cells were serum-starved for 3 h, and DHA, 4-HHE, and 4-HNE at different concentrations were added for 1 h. After incubation for 16 h, cells were taken for viability assay by incubating with the WST-1 reagent at 37 °C for 30 min. Color density was read at 450 nm with the plate reader. Results were obtained from triplicate assay from a single passage and expressed as mean ± SE (n = 3). Repeated experiment with different passages showed similar results. Analysis by one-way ANOVA followed by Bonferroni post-tests; “a” represents significant differences (p < 0.05) comparing test compounds with control

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