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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Unveiling anti-oxidative and anti-inflammatory effects of docosahexaenoic acid and its lipid peroxidation product on lipopolysaccharide-stimulated BV-2 microglial cells

Fig. 3

Effects of DHA, 4-HHE, and 4-HNE on LPS-induced ROS production in microglial cells. Cells were cultured in 96-well plates as described in Fig. 2 and harvested at 12 h after LPS treatment. For ROS determination, CM-H2DCFDA was added 1 h prior to the end of incubation. Data in ac represent levels of ROS after pre-treatment of DHA (12.5–100 μM), 4-HHE (1.25–10 μM), and 4-HNE (1.25–10 μM) in the presence and absence of LPS. Results are expressed as the mean ± SEM (n = 4). Analyzed by one-way ANOVA followed by Bonferroni post-tests; “a” represents significant differences (p < 0.05) comparing test compounds with control (Ctrl) with LPS treatment alone. IC50 values for each test compound were determined using the formula for regression analysis in Microsoft Excel 2016

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