Fig. 2

Fibronectin aggregates and plasma fibronectin shift microglia and bone marrow-derived macrophage morphologies towards amoeboid. Microglia (a–c) or bone marrow-derived macrophages (BMDMs, d, e) were left unstimulated (ctrl), cultured on plasma fibronectin (pFn) or fibronectin aggregates (aFn), or treated with interferon-γ (IFNγ) or interleukin-4 (IL-4). After 24 h, microglia (a–c) or BMDMs (d, e) were immunostained with isolectin-B4 (IB4), and morphologies of 100 cells per condition were scored as “amoeboid,” “ramified,” or “other,” with examples of control microglia shown in a. Dashed horizontal lines represent values of control cells set at 100% for each independent experiment. Note that the proportion of microglia and BMDMs with an amoeboid morphology was promoted by IFNγ treatment, pFn or aFn coatings relative to control cells. Bars represent mean values of each condition relative to control cells (set at 100% for each independent experiment, horizontal line) from four independent experiments. Error bars show the standard error of the mean. Statistical analyses were performed using logistic regression for each experiment separately, and representative statistical outcomes are summarized in the graph (*p < 0.05). Scale bar is 10 μm