Fig. 8

Hsp70 and thrombospondin-1 are present in MS lesion-derived fibronectin aggregates. Homogenates of human control white matter (n = 9), (chronic) active MS lesions ((c)sMS, n = 8) and chronic inactive MS lesions (ciMS, n = 9) were subjected to deoxycholate (DOC) (in)solubility assays (a–g). Hsp70 (a–c) and thrombospondin-1 (TSP1, d–g) levels in DOC-insoluble (I, containing Fn aggregates) and DOC-soluble (S, containing Fn dimers) fractions were analyzed by Western blot under non-reducing conditions. Note that TSP1 is enriched in (c)aMS lesions compared to CWM and ciMS lesions (d–g), while Hsp70 is equally present (a–c). In addition, TSP1 is tightly associated with the aggregates (agg, d, e). Box plots represent arbitrary intensity values of each condition. Statistical analyses were performed using an one-way ANOVA, followed by a Newman-Keuls Multiple Comparison test (*p < 0.05, **p < 0.01)