Fig. 9From: Fibronectin aggregates promote features of a classically and alternatively activated phenotype in macrophagesHsp70 increases iNOS expression, while both Hsp70 and thrombospondin-1 induce arginase-1 expression by bone marrow-derived macrophages. Bone marrow-derived macrophages (BMDMs, a–d) were left unstimulated (ctrl), cultured on fibronectin aggregates (aFn) or thrombospondin-1 (TSP1) or treated with Hsp70. Then, the expression of iNOS (a, b) and arginase-1 (c, d) was analyzed by Western blotting. Note that both aFn and Hsp70 increase iNOS and arginase-1 expression, while TSP1 induced only arginase-1 expression. Bars represent mean values of each condition relative to control cells (set at 1 for each independent experiment, horizontal line) from four independent experiments. Error bars show the standard error of the mean. Statistical analyses were performed using the one-sample t test when compared to control (*p < 0.05)Back to article page