Fig. 2

Effect of ethanol on MCP-1 and CCR2 expression. a C57BL6 mice of postnatal day 4 (PD4) were exposed to ethanol (EtOH) as described in the “Methods” section. 8 h after EtOH exposure, mice were sacrificed and the brain was processed for immunoblotting (IB) analysis of MCP-1 and CCR2. The expression of MCP-1, MCPIP, and CCR2 in the brain was quantified and normalized to actin. Each data point was the mean ± SEM of six animals. b The expression of MCP-1 and CCR2 mRNA in the brain was determined by quantitative RT-PCR as described in the “Methods” section. Each data point was the mean ± SEM of three animals. ***p < 0.001, statistically significant difference from control group. c SIM-A9 cells were exposed to EtOH (0.4%) for 3, 6, 12, or 12 h. The expression of MCP-1 and CCR2 was determined by IB and quantified by the normalization to the expression of actin. Each data point was the mean ± SEM of three independent experiments. *p < 0.05, statistically significant difference from control group