Fig. 4

Effect of ethanol on the developing brain of wild-type, MCP-1^−/−, and CCR2^−/− mice. Wild-type (WT), MCP-1^−/−, and CCR2^−/− mice of PD4 were exposed to EtOH for 8 h. a The expression of cleaved caspase-3 in the cerebral cortex (CTX) and the cerebellum (CB) was examined by immunohistochemistry (IHC) as described in the “Methods” section. Bar = 100 μm. b The expression of cleaved caspase-3 in the CTX and CB was determined by IB and quantified by the normalization to the expression of actin. Each data point was mean ± SEM of 3–5 animals **p < 0.01, *p < 0.05, statistically significant difference from WT group. c The degenerating neurons in the visual cortex were demonstrated by Fluoro-Jade C staining as described in the “Methods” section. Bar = 100 μm