Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Role of MCP-1 and CCR2 in ethanol-induced neuroinflammation and neurodegeneration in the developing brain

Fig. 6

Effect of Bindarit and RS504393 on ethanol-induced microglial activation in the developing mouse brain. Bindarit (100 mg/kg) or RS504393 (1 mg/kg) was administered by two subcutaneous (SC) injections at 24 and 0.5 h prior to EtOH exposure as described above. 8 h after EtOH injection, the pups were sacrificed and the brains were harvested. A representative image of inferior colliculus is shown. Microglia were identified by IHC of Iba-1. The resting microglia exhibit long branching processes and a small cellular body, which are indicated by arrow heads; while the activated microglia have fewer but thicker processes with a larger cell body, which are indicated by arrows. The percentage of activated Iba-1 positive cells in total Iba-1 positive cells was calculated as described in the “Methods” section. Bar = 50 μm. Each data point was the mean ± SEM of three animals. *p < 0.05, statistically significant difference from control group, #p < 0.05, statistically significant difference from EtOH-treated group

Back to article page