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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition

Fig. 1

Proteomic analysis of synaptosomes from Toxoplasma-infected mice. Synaptosomes from infected and non-infected mice were isolated, proteins were digested, and peptides were iTRAQ labeled and analyzed using mass spectrometry. a Heatmaps showing relative protein abundances (log2) of the top 100 significantly upregulated (left) and downregulated (right) proteins in synaptosomes after T. gondii infection in four separate sample pairs. Color codes are indicated. b Boxplots showing regulations (log2) of protein abundances from infected and non-infected samples from four separate sample pairs (1–4) and from infected (inf/inf) and non-infected (non/non) synaptosomal fractions from one experiment. c Single enrichment analysis of the up- and downregulated proteins. The y-axis denotes the number of proteins, which were assigned to the KEGG- or GO annotation; upregulated functions or pathways are depicted in green; downregulated functions or pathways in red. Only significant enriched annotations from KEGG and from annotations related to “biological process” and “molecular function” from Gene Ontology database are depicted. The values on top of the bars denote the p values. The GO annotation “antigen processing via MHC class I” stands for “antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent.” The KEGG annotation “protein processing in ER” stands for “protein processing in endoplasmic reticulum”

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