Fig. 4

Glutamatergic signaling is affected upon chronic Toxoplasma infection. The coronal sections of Toxoplasma gondii-infected (INF) and control animals (CTRL), stained with markers of glutamatergic synapses, the postsynaptic scaffolding protein Shank3 and the glutamate transporter EAAT2, with focus on the specific cortical and subcortical areas (neocortex, thalamus, striatum). a Composed tile scan images with × 10 objective of whole brain and × 63 objective of somatosensory cortical areas (b). Integrated density (c) from each quantified area was normalized to hypothalamic reference area (see Additional file 2), indicating decreased cortical staining for EAAT2. Control mice (CTRL) n = 3, infected mice (INF) n = 3. Scale bars (a) 1 mm, scale bars (b) 40 μm. Displayed results are mean values ± SEM. *p < 0.05