Fig. 1

The spleen is a site of cervical SCI-induced inflammation within hours of trauma. Splenic tissue was collected at 1 and 24 h following cervical SCI and laminectomy-only surgeries. Cytokines were assessed using the rat cytokine R&D ELISA Proteome Profiler array (ARY008), and a representative heat map was generated using the BROAD Institute’s R implementation of Morpheus with Euclidean distance hierarchical clustering (a). Data are expressed as mean Log2 (fold change) relative to aged-matched naive spleen tissue (n = 4 per group). Statistically significant differences in cytokine expression (multiple t tests with Holm-Sidak correction for multiple comparisons) are shown at 1 h (b) and 24 h (c) following SCI between time-matched laminectomy-only surgery and injured groups