Fig. 2

Acute spinal cord parenchymal hemorrhage and lesion volume is unaffected by splenectomy at 1, 3, or 7 days following SCI. Schematic of experimental design for acute assessments (a). In short, splenectomies were performed 4 days prior to clip-compression C7-T1 SCI in Wistar rats. Drabkin’s assay was applied to quantify parenchymal hemorrhage at 1 (n = 5 per group), 3 (n = 7 per group), and 7 (n = 5 per group) days post-SCI between the animals with or without a spleen (b). Very high-resolution ultrasound (VHRUS) imaging in B mode was also applied to quantify lesion volume (c). The representative sagittal view VHRUS images are shown at 1, 3, and 7 days post-SCI (d). Data are expressed as mean ± SEM, and unpaired t tests were performed at each time point with no significant differences found