Fig. 2

miR-155 expression in TLE-HS. a miR-155 expression was increased in TLE-HS patients relative to controls evaluated by TaqMan RT-qPCR (p < 0.001). b–e In situ hybridization for miR-155 in the human hippocampus. A moderate neuronal miR-155 expression was observed in the control hippocampus (b, c). d, e miR-155 expression was increased in both neurons and in cells with glial morphology compared to control; co-localization was found with neurons (NeuN; d, inset a), astrocytes (GFAP; d, inset b), endothelial cells (CD34; e, inset c) and microglia (CR3/43; e, inset d). Arrowheads indicate cells with glial morphology. f–h A semi-quantitative analysis of miR-155 IRS in neurons, cells with glial morphology, and blood vessels. Scale bar 100 μm; *p < 0.05, **p < 0.01, ***p < 0.001, Mann-Whitney U test, error bars depict standard error of the mean