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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: The novel estrogenic receptor GPR30 alleviates ischemic injury by inhibiting TLR4-mediated microglial inflammation

Fig. 4

Activating GPR30 in microglia reduces neuronal injury. a Neurons and microglia were co-cultured for 2 days and then separated; then, the microglia were subjected to OGD treatment for 4 h and treated with drugs, including E2 (10 nM), G1 (10 nM), and ICI182780 (1 μM), immediately upon reintroduction for 1 h. From the time the microglia received the drug treatments, the neurons were subjected to OGD treatment for 1 h. Then, the neurons and microglia were co-cultured again in new medium for 12 h. CCK-8 cell viability and LDH release assays were performed to detect cell injury. b Activation of GPR30 in microglia by E2 and G1 significantly improved the cell viability of neurons. The data are expressed as the mean ± SD and analyzed by one-way ANOVA with Tukey’s post-test. ###p < 0.001 compared with the con group. *p < 0.05, **p < 0.01 compared with the vehicle group. The data were pooled from six independent experiments. c Activation of GPR30 in microglia by E2 and G1 significantly reduced LDH release, indicating that the cell injury was alleviated. The data are expressed as the mean ± SD and analyzed by one-way ANOVA with Tukey’s post-test. ###p < 0.001 compared with the con group. *p < 0.05, **p < 0.01 compared with the vehicle group. The data were pooled from six independent experiments. LDH lactate dehydrogenase

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