Fig. 1
Aging led to decreased adhesion and migration of microglia to Aβ fibrils. Microglia from mice of various ages were isolated, and the effects of adhesion and migration of microglia to fAβ were determined using adhesion and migration assays. a, b The adhesion of microglia to fAβ. Microglia isolated from neonatal, 3-month-old, 15-month-old WT mice or 3-month-old, and 15-month-old APP/PS1 mice were plated on spots of BSA (0.01% in PBS), fAβ1–42 (10 μM) or fAβ42–1 (10 μM) for 24 h. The numbers of cells within spot areas were quantified. a WT mice. b APP/PS1 mice. c, d The migration of microglia to fAβ. Microglia isolated from neonatal, 3-month-old, 15-month-old WT mice or 3-month-old, and 15-month-old APP/PS1 mice were added to transwell membranes coated on their underside with BSA (0.01% in PBS), fAβ1–42 (10 μM), or fAβ42–1 (10 μM) for 24 h. The numbers of cells that transmigrated through the transwell membranes were quantified. c WT mice. d APP/PS1 mice. Values were reported as the mean ± SD, as a percentage of values determined in BSA group of neonatal mice (control, 100%). *p < 0.05, **p < 0.01, ***p < 0.001, when compared with the BSA group; $p < 0.05, $$$p < 0.001, n = 3