Fig. 3
NgR mediated the effects of Nogo-P4 on adhesion and migration of adult microglia to fAβ. a, b NgR mediated the effect of Nogo-P4 on adhesion of adult microglia to fAβ1–42. Before adding to wells pre-coated with BSA (0.01% in PBS), fAβ1–42 (10 μM), or Nogo-P4 (100 μg/ml) + fAβ1–42 (10 μM), microglia from 3-month-old mice were pretreated with NEP1-40 (10 μM) or PI-PLC (0.3 U/ml) for 30 min to interrupt the function of NgR. The numbers of cells within spot areas were quantified. c, d NgR mediated the effect of Nogo-P4 on migration of adult microglia to fAβ1–42. Before adding into the transwells pre-coated with BSA (0.01% in PBS), fAβ1–42 (10 μM), or Nogo-P4 (100 μg/ml) + fAβ1–42 (10 μM), microglia from 3-month-old mice were pretreated with NEP1-40 (10 μM) or PI-PLC (0.3 U/ml) for 30 min to interrupt the function of NgR. The cell numbers transmigrated through transwell membranes were quantified. Values were reported as the mean ± SD, as a percentage of values determined in BSA group (control, 100%). *p < 0.05, **p < 0.01, ***p < 0.001, when compared with the BSA group; ###p < 0.001, when compared with the Nogo-P4 + fAβ1–42 group, n = 3