Fig. 4
Rho GTPases were involved in effects of Nogo-P4 on microglia adhesion and migration to fAβ. a, b The activation of RhoA in microglia was determined using a Rho Activation Assay Kit after microglia from 3-month-old mice were stimulated with BSA (0.01% in PBS), fAβ1–42 (10 μM), Nogo-P4 (100 μg/ml), or Nogo-P4 (100 μg/ml) + fAβ1–42 (10 μM) for 6 h. Values were reported as the mean ± SD. *p < 0.05, **p < 0.01, when compared with BSA group, n = 3. c–f RhoA/ROCK pathway involved in the regulation of Nogo-P4 on adhesion and migration of microglia from 3-month-old mice to Aβ fibrils. Quantitative assessment of cell adhesion (c, d) and migration (e, f) by determining the cell numbers, after pre-treatment with Y27632 (50 μM) for 30 min. Values were reported as the mean ± SD, as a percentage of values determined in BSA group (control, 100%). **p < 0.01, ***p < 0.001, when compared with the BSA group; ##p < 0.01, when compared with the Nogo-P4 + fAβ1–42 group, n = 3. The activation of Rac1 (g, h) and Cdc42 (i, j) in microglia were determined using a Rac1/Cdc42 Activation Assay Kit. Microglia from 3-month-old mice were stimulated with BSA (0.01% in PBS), fAβ1–42 (10 μM), Nogo-P4 (100 μg/ml), or Nogo-P4 (100 μg/ml) + fAβ1–42 (10 μM) for 6 h. Values were reported as the mean ± SD. **p < 0.01, ***p < 0.001, when compared with the BSA group; ###p < 0.001, when compared with the fAβ1–42 group, n = 3