Fig. 1

Experimental flowchart. Pregnant C57BL/6 mice were treated with influenza vaccine (VAC) or sterilized PBS at E2.5 and with sterilized LPS or PBS at E14.5. Two hours after the LPS or PBS injection at E14.5, maternal serum corticosterone and IL-17a levels were detected. The fetal brains were removed for morphology, protein, and gene detection at E18.5. The offspring were submitted to behavior tests at 4 weeks (P28) and 8 weeks (P56) after birth, and the brains were removed for immunofluorescence staining at 6 weeks (P42)