Fig. 2
From: Levels of ADAM10 are reduced in Alzheimer’s disease CSF
Characterization of CSF-ADAM10 complexes by sucrose gradient ultracentrifugation. CSF samples (NADC) were fractionated on 5–20% sucrose density gradients. The fractions (collected from the top of each tube) were immunoblotted using the ectodomain antibody specific for a domain common to all the soluble CSF-ADAM10 species. Enzymes of known sedimentation coefficient, alkaline phosphatase (P, 6.1S; ~ 140–160 kDa), catalase (C, 11.4S; ~ 232 kDa), and β-galactosidase (G, 16.0S; ~ 540 kDa) were used as internal markers. A representative case from three independent experiments is shown