Fig. 2

hrIL-1Ra titers within tissues of interest from pups exposed to LPS+HI+IL-1Ra±HT. hrIL-1Ra titers measured by ELISA at 24 h post-HI were increased within the plasma (a), right cerebral hemisphere (b), and CSF (c) in LPS+HI+HT+IL-1Ra (50 mg/kg) as compared to LPS+HI+IL-1Ra (50 mg/kg) condition. The concentrations of hrIL-1Ra were similar in both conditions at 4 h post-HI, as well as at 24 h post-HI within the liver (d). The number (n) of rats used was LPS+HI+IL-1Ra (n = 5–8 from 4 litters) and LPS+HI+HT+IL-1Ra (n = 5–8 from 4 litters). The bars indicate the mean ± SEM. *p ≤ 0.05, **p ≤ 0.01; independent T test. Abbreviations: CSF, cerebrospinal fluid; HI, hypoxia-ischemia; HT, hypothermia; hrIL-1Ra, human recombinant of interleukin-1 receptor antagonist; LPS, lipopolysaccharide from Escherichia coli