Fig. 3

IL-1β expression within tissues of interest from pups exposed to LPS+HI+IL-1Ra±HT. IL-1β concentration measured by ELISA at 4 h and 24 h post-HI within the plasma (a), right cerebral hemisphere (b), and liver (c) in LPS+HI+IL-1Ra (50 mg/kg) and LPS+HI+HT+IL-1Ra (50 mg/kg) conditions. HT increased the expression of IL-1β within the right hemisphere at 4 h post-HI (b). The number (n) of rats used was LPS+HI+IL-1Ra (n = 5–7 from 4 litters) and LPS+HI+HT+IL-1Ra (n = 4–8 from 4 litters). The bars indicate the mean ± SEM. *p ≤ 0.05; independent T test. Abbreviations: HI, hypoxia-ischemia; HT, hypothermia; hrIL-1Ra, human recombinant of interleukin-1 receptor antagonist; IL-1β, interleukin-1β; LPS, lipopolysaccharide from Escherichia coli