Skip to main content
Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Trapped in the epineurium: early entry into the endoneurium is restricted to neuritogenic T cells in experimental autoimmune neuritis

Fig. 1

No differences of EAN disease course and T cell infiltration of the sciatic nerve with neuritogenic T cells expressing GFP or non-GFP-expressing control T cells. a Clinical course of the disease of animals used for the analysis of adoptive transfer of CD4P2-GFP T cells. Three independent experiments with n = 2–4 rats at each time point (n = 59 in total). Neuritogenic T cells without GFP expression served as a control (n = 10). Clinical scoring was assessed daily using the following system: 0 no impairment, 1 reduced tail tone, 2 limp tail, 3 absent righting reflex, 4 gait ataxia, 5 mild paraparesis, 6 moderate paraparesis, 7 severe paraparesis or paraplegia, 8 tetraparesis, 9 moribund, and 10 death due to neuropathy. Depicted are clinical score means ± SEM for each day. At no time point, a statistical significance was detected (p > 0.5; Wilcoxon-Mann-Whitney test). b Gated on lymphocytes, frequencies of T cells were determined after adoptive transfer of GFP-transduced (CD4P2-GFP) and non-transduced neuritogenic control cells (CD4P2) in the sciatic nerve at day seven. Representative flow cytometry staining of CD4 and CD8 T cells gated on CD45+ cells are shown on the left side. Upper panel shows T cell composition after transfer of CD4P2 control cells; lower panel shows the results of GFP-expressing neuritogenic T cells (CD4P2-GFP). Numbers indicate the percentages of CD4 T cells within the lymphocyte gate. On the right, representative dot plots from CD4 staining and GFP expression (measured in FITC channel) gated on CD45+ cells are shown. Numbers indicate the percentages of GFP+ T cells within the lymphocyte gate

Back to article page