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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Trapped in the epineurium: early entry into the endoneurium is restricted to neuritogenic T cells in experimental autoimmune neuritis

Fig. 2

Flow cytometry analysis of kinetics of GFP-expressing neuritogenic T cells in different organs after AT-EAN. Flow cytometry analysis of the percentages of T cells (blue panel) and GFP-expressing neuritogenic T cells (green panel) in the different organs at the indicated time points (days one to six) after adoptive transfer of CD4P2-GFP gated on lymphocytes. Number of T cells was calculated based on the overall number of lymphocytes in the sciatic nerve determined by flow cytometry. GFP expression was detected in FITC channel. a Percentage of T cells and neuritogenic GFP-expressing in the spleen, draining lymph node (dLN), lung, liver, and blood. Depicted is mean ± SEM. Asterisks indicate significant differences compared to day one (*p < 0.05, **p < 0.01, ***p < 0.001; Student’s t test). b Percentage and number of T cells and neuritogenic GFP-expressing in the sciatic nerve. Note that the preparation method of the sciatic nerve cannot differentiate between endoneurium, perineurium, and epineurium. Depicted is mean ± SEM. Asterisks indicate significant differences compared to day one (*p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test). c Percentage of T cells (blue bars) and GFP-expressing neuritogenic T cells (green bars) on the left y axis and the clinical score (orange) on the right y axis at the indicated time points (days one to six). To simplify the graph, only the mean is depicted

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