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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Trapped in the epineurium: early entry into the endoneurium is restricted to neuritogenic T cells in experimental autoimmune neuritis

Fig. 3

Kinetics of GFP-expressing neuritogenic T cells in the endoneurium after AT-EAN. Histological analysis of the number of T cells (blue) and GFP-expressing neuritogenic T cells (green) in the sciatic nerve endoneurium after adoptive transfer of CD4P2-GFP at the indicated time points. T cells were counted in cross sections of five random images in four different regions (from proximal to distal) of sciatic nerves from animals of independent experiments (n = 4). Cross sections were stained with antibodies against either CD4 or GFP. Cell nuclei were visualized using DAPI. Note that the number of GFP-positive cells is as high as the number of CD4 T cells (GFP vs. CD4 p > 0.5 for each time point, unpaired Student’s t test). Representative images used for counting are shown in Fig. 4a. Depicted are mean positive cells/area ± SEM. Asterisks indicate significant differences compared to day one (*p < 0.05, **p < 0.01, ***p < 0.001, Student’s t test)

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