Fig. 4
From: Metalloprotease Adam10 suppresses epilepsy through repression of hippocampal neuroinflammation
Adam10 overexpression decreases spontaneous seizures in TLE mice. a Schematic diagram of the experimental design. Mice were bilaterally injected with the virus into the hippocampus, and after 2 weeks of recovery, these mice were induced for SE and continuously video EEG monitored for 4 weeks for SE and SRS analysis. These mice were then sacrificed after the EEG recording was completed at day 28 post-SE to detect hippocampal neuroinflammation. b A typical EEG recording of the baseline and seizure in the cortex and hippocampus. c Bar graph showing the average time to onset of SE in the Vehicle Ctrl, AAV-Ctrl, and AAV-Adam10 mice (n = 12). d Bar graph showing the quantification of SE duration in the Vehicle Ctrl, AAV-Ctrl, and AAV-Adam10 mice (n = 10). e Line graphs showing the seizure severity during SE development in the Vehicle Ctrl, AAV-Ctrl, and AAV-Adam10 mice (at 15–30 min, F2,27 = 14.02, p = 0.004, AAV-Adam10 vs Vehicle Ctrl; p < 0.001, AAV-Adam10 vs AAV-Ctrl; at 30–45 min, F2,27 = 7.41, p = 0.032, AAV-Adam10 vs Vehicle Ctrl; p = 0.026, AAV-Adam10 vs AAV-Ctrl) (n = 10). f Bar graph showing the average time to onset of first spontaneous seizure in the Vehicle Ctrl, AAV-Ctrl, and AAV-Adam10 mice (n = 6). g Bar graph showing the SRS frequency in the Vehicle Ctrl, AAV-Ctrl, and AAV-Adam10 mice (F2,15 = 4.23, p = 0.031, AAV-Adam10 vs AAV-Ctrl) (n = 6). h Bar graph showing the quantification of electrographic SRS duration in the Vehicle Ctrl, AAV-Ctrl, and AAV-Adam10 mice (n = 6). *p < 0.05 and one-way ANOVA