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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Metalloprotease Adam10 suppresses epilepsy through repression of hippocampal neuroinflammation

Fig. 6

shRNA-mediated Adam10 knockdown in the hippocampus. a Action mode of shRNA-mediated Adam10 knockdown via lentiviral vector infection. b Distribution of lentivirus-mediated cop-GFP expression in the CA1 region of the hippocampus. c, d RT-PCR analysis of Adam10 expression in the hippocampus of Vehicle Ctrl, shRNA-Ctrl, and shRNA-Adam10 mice, respectively (F2,12 = 8.84, p = 0.008, shRNA-Adam10 vs Vehicle Ctrl; p = 0.032, shRNA-Adam10 vs shRNA-Ctrl) (n = 5). e, f Western blot analysis of Adam10 protein levels in the hippocampus of Vehicle Ctrl, shRNA-Ctrl, and shRNA-Adam10 mice, respectively (F2,12 = 6.38, p = 0.021, shRNA-Adam10 vs Vehicle Ctrl; p = 0.027, shRNA-Adam10 vs shRNA-Ctrl) (n = 5). g Representative images of the immunostaining of Adam10 in the hippocampal CA1 region of the shRNA-Ctrl and shRNA-Adam10 mice, respectively. h Bar graph showing the quantification of Adam10-positive cells in the hippocampal CA1 region of shRNA-Ctrl and shRNA-Adam10 mice, respectively (p = 0.014) (n = 4). *p < 0.05, **p < 0.01, unpaired two-tailed Student’s t test, and one-way ANOVA. Scale bar = 50 μm in b and 20 μm in g

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