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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo

Fig. 4

Microglia polarization kinetics. *p < 0.05, **p < 0.01, ***p < 0.001 compared to control; #p < 0.05 compared to different experimental group as marked by horizontal bar; only relevant significant values are highlighted. a Transient exposure to an acute inflammatory stimulus (24 h, followed by media change) of 10 ng/ml LPS (left and middle panel, red vertical bars) or 50 ng/ml IL4 (right panel, green vertical bar). INOS expression as a function of time after transient exposure was measured on the protein level by immunocytochemistry (n = 3, H(4) = 94.638, p < 0.001), NO release by Griess assay (μmol/l; n = 7, H(4) = 32.616, p < 0.001), and IGF1 release by ELISA (pg/ml, n = 5, H(4) = 10.128, p < 0.05). Data were normalized to control. b Permanent exposure to a chronic inflammatory stimulus, applied over the entire observation period of 96 h, of 10 ng/ml LPS (left and middle panel, red horizontal bars) or 50 ng/ml IL4 (right panel, green horizontal bar). INOS expression as a function of time after the beginning of permanent exposure was measured on the protein level by immunocytochemistry (n = 3, H(4) = 95.861, p < 0.001), NO release was measured by Griess assay (μmol/l; n = 7, H(4) = 44.542, p < 0.001); IGF1 release was measured by ELISA (pg/ml, n = 5, F (4, 27) = 259.4, p < 0.001, ω = 0.98). Data were normalized to control

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