Skip to main content
Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo

Fig. 7

Effects of M1- and M2-conditioned microglia media on endogenous NSCs in vivo. #p < 0.05, ##p < 0.01, ###p < 0.001 compared to different experimental group as marked by horizontal bar. Male adult Wistar rats received a single intracerebroventricular (i.c.v.) injection of M1-conditioned medium (n = 5), M2-conditioned medium (n = 5), or regular culture medium as control (n = 4). All animals received bromodeoxyuridine (BrdU) over 5 days and were sacrificed after 8 days for immunohistochemistry. a Effects of microglia-conditioned media on neurogenesis in the subventricular zone (SVZ) as assessed by staining for doublecortin (DCX)-positive neuroblasts (F(2, 19) = 14.215, p < 0.001, ω = 0.74). Representative images of DCX-immunohistochemistry (red) with Hoechst (blue) as nuclear counterstain; scale bar = 100 μm; in the magnified insert 20 μm. b Effects of microglia-conditioned media on proliferation in the SVZ as assessed by staining for BrdU (F(2, 19) = 2.443, p < 0.1, ω = 0.34). Representative images of BrdU immunohistochemistry (green); scale bar = 100 μm; in the magnified insert 20 μm. c Effects of microglia-conditioned media on the generation of astrocytes in the striatum directly adjacent to the SVZ (H(2) = 7.936, p < 0.05). Representative images of GFAP immunohistochemistry (red) with Hoechst (blue) as nuclear counterstain; scale bar = 50 μm

Back to article page