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Table 1 Used primers and parameters of RT-qPCR

From: The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo

RNA

Sequences forward/backward 5′-3′

Temperature (°C) step 1/2/3

Duration (s) step 1/2/3

Accession number

iNOS

GCTTGTCTCTGGGTCCTCTG/CTCACTGGGACAGCACAGAA

95/59/72

15/15/45

NM_012611.3

CD206

AACAAGAATGGTGGGCAGTC/CCTTTCAGTCCTTTGCAAGC

95/56/72

15/15/45

NM_001106123.2

Ki67

TCTTGGCACTCACAGTCCAG/ GCTGGAAGCAAGTGAAGTCC

95/58/72

15/15/45

NM_001271366.1

RPL13a

TCTCCGAAAGCGGATGAACAC/CAACACCTTGAGGCGTTCCA

  

NM_173340.2

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Journal of Neuroinflammation

ISSN: 1742-2094