Fig. 2
K284-6111 on Aβ1–42-infused mice in both GFAP and IBA-1-positive mice brain. Immunostaining of CHI3L1, iNOS, GFAP, and Iba-1 proteins in the hippocampus were performed in 20-μm-thick sections of mice brain with specific primary antibodies and biotinylated secondary antibodies (a, b). The expression of CHI3L1, GFAP, Iba-1, COX-2, and iNOS were detected by Western blotting using specific antibodies in the mice brain. For the cropped images, samples were run in the same gels under the same experimental conditions and processed in parallel. Band density was quantified from three mice (c). mRNA levels of CHI3L1 (d), TNF-α (e), IL-1β (f), and IL-6 (g) were detected by qRT-PCR in hippocampus. #Significant difference to control mice (P < 0.05), *Significant difference to Aβ1–42-injected mice (P < 0.05)