Fig. 3
K284-6111 inhibits accumulation of Aβ1–42 in the brain of Aβ1–42-infused mice brain. a Aβ accumulation in the brains of Aβ1–42-infused mice was determined by thioflavin S staining. The levels of Aβ1–42 in mice brain (n = 5) were measured by ELISA (b). The activity of β-secretase in mice brain (n = 5) was investigated using an assay kit (c). The expression of APP, BACE1, and C99 were detected by Western blotting using specific antibodies in the mice brain (d). For the cropped images, samples were run in the same gels under the same experimental conditions and processed in parallel. Band density was quantified from three mice (d). #Significant difference to control mice (P < 0.05), *Significant difference to Aβ1–42-injected mice (P < 0.05)