Fig. 4
K284-6111 on NF-κB translocation related DNA-binding activity-mediated CHI3L1. Phosphorylation of IκB, and p50 and p65 translocation was detected by Western blotting using specific antibodies in mice brain. β-actin and HistoneH1 protein was used as an internal control, and the graphs represented the arbitrary density of blot signal (a). Band density was quantified from three mice (a). Whole cell lysates of B16F10 were incubated with K284-6111-conjugated Sepharose 4B. After precipitation, the levels of bound CHI3L1 were monitored by Western blot analysis (b). Docking model of K284-6111 (c). CHI3L1 siRNA augmented K284-6111-induced inhibitory effect on NF-κB expression and inflammation (d). Each value is mean ± S.D. from three experiments (d). *Significant difference from control group (p < 0.05). # Significant difference from Aβ1–42-infused group (p < 0.05)