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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Inhibiting aberrant p53-PUMA feedback loop activation attenuates ischaemia reperfusion-induced neuroapoptosis and neuroinflammation in rats by downregulating caspase 3 and the NF-κB cytokine pathway

Fig. 4

Effect of intrathecal si-PUMA and PFT-α injections on PUMA and p53 expression at 48 h after IR. a Representative western blots of mitochondrial and cytosolic PUMA protein levels at 48 h post-IR. COX-IV and β-actin were used as loading controls. b Quantification of the relative protein levels of PUMA in the mitochondria and cytoplasm. c Quantification of the relative mRNA expression of PUMA. d Representative western blots of nuclear and cytosolic p53 protein levels at 48 h post-IR. Histone and β-actin were used as loading controls. e Quantification of the relative protein levels of p53 in the nucleus and cytoplasm. f Quantification of the relative mRNA expression of p53. All data were obtained from three independent experiments and expressed as the mean ± SEM. *P < 0.05, versus the sham group. *P < 0.05, versus the IR group. *P < 0.05, versus the si-PUMA group

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