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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Inhibiting aberrant p53-PUMA feedback loop activation attenuates ischaemia reperfusion-induced neuroapoptosis and neuroinflammation in rats by downregulating caspase 3 and the NF-κB cytokine pathway

Fig. 6

Effects of si-PUMA and PFT-α on NF-κB translocation and proinflammatory cytokine release after IR. a Representative immunofluorescence of the colocalization of p53 (green) and NF-κB (red) in the anteriorl horns of grey matter at 48 h after IR. The arrows indicate co-localization with yellow labelling. Scale bars = 50 μm. b and c Quantitation of p53 and NF-κB immunoreactivity in the anteriorl horns of grey matter at 48 h post-IR. d Quantification of p53/NF-κB double-positive cells is presented as the average of three independent images. e Representative western blots of the nuclear protein and cytosolic phosphorylation levels of NF-κB at 48 h post-IR. Histone and β-actin were used as loading controls. f Quantification of the relative protein levels of NF-κB in the nucleus and cytoplasm. g and h Quantification of spinal cord IL-1β and TNF-α concentrations at 48 h after IR. Data are expressed as the mean ± SEM. *P < 0.05, versus the sham group. *P < 0.05, versus the IR group

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