Fig. 2
From: Blockade of MCAM/CD146 impedes CNS infiltration of T cells over the choroid plexus
MCAM and VLA4 blockade in a model of spontaneous EAE. Development of spontaneous EAE-like disease in Devic mice that were either treated with (a) anti-VLA4 (clone PS/2), (b) anti-MCAM (clone 15), (c) anti-MCAM and anti-VLA4 antibodies, or the respective isotype control antibodies. Mice were treated every second day from day 16 after birth. Littermates were used for the treatment with either the respective blocking antibody or the isotype control. Mean clinical EAN scores ± SEM of three independent experiments over time are shown; *P < 0.05; green highlighted areas: P < 0.1. (d) Immunofluorescence staining of MCAM (green) in choroid plexus tissue obtained from Devic mice treated with anti-VLA-4, anti-MCAM, or anti-MCAM/VLA-4 neutralizing antibodies. Nuclear staining (DAPI) is shown in blue. Scale bars are 10 μm