Fig. 3

Laminin α4 localization to choroid plexus endothelial basement membranes. C57BL/6 mouse choroid plexus samples immunofluorescently stained for (a) laminin α4 (green) and MECA32 antigen as an endothelial cell marker (red) or (b) laminin α4 (red) and plectin to mark choroid epithelium (green), demonstrating colocalization of laminin α4 with the MECA32⁺ blood vessels located beneath the epithelial layers. Cell nuclei are counterstained with DAPI (blue). Higher magnifications of the boxed areas are shown in the lower panels. Bars are 100 μm (upper panels) and 10 μm (lower panels), respectively. c–e Immunofluorescence staining of choroid plexus explants of C57BL/6 mice for (c) laminin α4 (red) and PECAM (green), (d) laminin α4 (green) and MCAM (red), and (e) PECAM (green) and MCAM (red). Nuclear staining (DAPI) is shown in blue, and left panels represent control stainings. Scale bars: 10 μm