Fig. 3

Relish signaling in astrocytes influences SCA3^polyQ78-induced gene expression but not the extent of SCA3 aggregation. a Analysis of the effect of modulating Relish expression in astrocytes or Relish heterozygosity on Relish activity in heads of flies expressing SCA3^polyQ78 in the eyes. Expression of Relish target genes (DptA or AttC) was determined in heads of control flies (control) and flies expressing SCA3^polyQ78 in the eyes. The effect of Relish RNAi targeted to astrocytes (Relish RNAi #1 and Relish RNAi #2), Relish overexpression or heterozygosity for Relish (Relish −/+) in flies expressing eye-specific SCA3^polyQ78 was determined. b Lysates of fly heads described in (a) were analyzed on Western blot to determine levels of soluble and aggregated HA-tagged SCA3^polyQ78. c Quantification of the SDS-soluble/SDS-insoluble ratio of SCA3^polyQ78 of three independent experiments shown in b. n.s., not significant. Genotypes in a and b: control, GMR-QF/+; for other genotypes, see Fig. 2b