Fig. 4From: Inhibition of NF-κB in astrocytes is sufficient to delay neurodegeneration induced by proteotoxicity in neuronsRelish target genes influence SCA3polyQ78-induced degeneration but not SCA3polyQ78 aggregation or SCA3polyQ78-induced relocation of astrocytes. a Representative images of flies expressing eye-specific SCA3polyQ78 compared to flies coexpressing RNAi constructs in astrocytes targeting Relish target genes AttA or CecA. b Quantification of the fraction of degeneration of the eyes shown in a. Data are representative of at least three independent experiments. c Representative images of the effect of downregulation of Relish target genes AttA or CecA in astrocytes on SCA3-induced degeneration or the relocation of astrocytes upon expression of eye-specific SCA3polyQ78 by using eye-specific expression of mCD8-GFP and astrocyte-targeted expression of myr-RFP (RFP). d Quantification of the effect of Relish target genes in astrocytes on SCA3-induced degeneration by using membrane-targeted mCD8-GFP. Lysates of fly heads expressing mCD8-GFP and SCA3polyQ78 in the eyes were analyzed on western blot and compared to flies coexpressing RNAi constructs targeting AttA or CecA in astrocytes. Quantifications are of at least three independent experiments. e No effect on Relish target genes on levels of astrocytes expressing myr-RFP. Lysates of fly heads of control flies or flies expressing myr-RFP in astrocytes together with SCA3polyQ78 in the eyes in the presence or absence or AMP RNAi constructs (AttA RNAi or CecA RNAi) were analyzed on western blot for expression for myr-RFP and tubulin. Quantifications are of two independent experiments. f Analysis of the effect of Relish target genes in astrocytes on SDS-solubility of SCA3polyQ78 expressed in the eyes. Head lysates of flies expressing HA-tagged SCA3polyQ78 in the eyes were compared to flies coexpressing RNAi constructs targeting AttA or CecA in astrocytes. Quantifications of the SDS-soluble/SDS-insoluble ratio are of at least three independent experiments. Genotypes a -: GMR-QF2/+; QUAS-SCA3polyQ78:: alrm-Gal4/+. AttA RNAi, GMR-QF2/+; QUAS-SCA3polyQ78:: alrm-Gal4/UAS-AttA RNAi. CecA RNAi, GMR-QF2/+; QUAS-SCA3polyQ78:: alrm-Gal4/+; UAS-CecA RNAi/+. c, d Con: GMR-QF2/+, the rest of the genotypes as in a but with coexpression of QUAS-mCD8-GFP or UAS-myr-RFP. e, f Con: GMR-QF2/+; other samples as in aBack to article page