Fig. 7

Effects of rh-TSG-6 on SAH-induced microglia polarization. a Representative FACS plots showing gating strategy we use in flow cytometry analysis. Cells populations in the right dot plots defined as CD11b⁺ CD45^low (microglia) were gated for further analysis. Representative dot plots showing the ratio of CD86-positive subsets (b) and CD163-positive subsets (c) in CD11b⁺ CD45^low populations in the sham group, SAH group, SAH + vehicle group and SAH + rh-TSG-6 group. Bar graphs show quantitative analysis of CD86-positive subsets of CD11b⁺ CD45^low populations (M1 microglia) (d) and CD163-positive subsets of CD11b⁺ CD45^low populations (M2 microglia) (e) in different groups. Inflammatory mediators expressed in the brain cortex after SAH were detected with ELISA. Tissue TNF-α and IL-10 concentrations were analyzed from samples and standards in duplicates and expressed as pictograms per milliliter. rh-TSG-6 administration obviously reduced the level of TNF-α (f) and increased the level of IL-10 (g) in the brain tissue. Samples for FACS and ELISA experiments sorted from tissue lysates of left temporal lobe of the brain. The values (means ± SD) are representative of three independent experiments. *p < 0.05. n = 6/group. SSC = side scatter channel, FSC-A = forward scatter channel area, FITC = fluorescein isothiocyanate, PE = phycoerythrin, and APC = allophycocyanin