Fig. 1

Anti-TLR2 antibody increases phagocytosis of Aβ. Microglia from neonatal mice were plated on poly-D-lysine-coated coverslips, incubated with LPS (100 ng/ml) for 4 h after which time anti-TLR2 antibody (2.5 mM/ml) was added. Aβ (10 μM) was added 4.5 h later and incubation continued for 24 h. Cells were harvested, fixed in 4% PFA and stained for Iba1 and Aβ as described in the “Methods” section. a Mean data revealed that incubation of LPS + Aβ-stimulated cells with anti-TLR2 antibody significantly increased phagocytosis of Aβ (**p < 0.01 LPS + Aβ+anti-TLR2 vs LPS + Aβ; Student’s t test for independent means; n = 3). Aβ uptake is expressed as a ratio between the number of Aβ⁺ Iba⁺ cells as a total number of Iba⁺ cells. A total of 10 fields per experiment in triplicate were analysed. b The panel shows confocal fluorescence images at × 63 magnification. The presence of Aβ (green) is evident in Iba1⁺ (red) cells that were incubated in LPS + Aβ with anti-TLR2 antibody. (Scale bar = 20 μm)