Fig. 2

Anti-TLR2 antibody increases the expression of Arg1 and LAMP1 and decreases Iba1 expression in microglia. Microglia were prepared and treated as described in the legend for Fig. 1 and stained for LAMP1, Arg1 and Iba1 as described in the “Methods” section. a The panel shows confocal fluorescence images at × 40 magnification and shows that Iba1 staining was increased in microglia incubated with LPS + Aβ. LAMP1 staining and Arg1 staining were increased in microglia that were incubated in LPS + Aβ+anti-TLR2 antibody. (Scale bar = 50 μm). b–d Analysis of the mean data indicate that incubation of LPS + Aβ-stimulated cells with anti-TLR2 antibody significantly increased LAMP1 staining (b) and Arg1 staining c, whereas the significant LPS + Aβ-induced increase in Iba1 (d) was attenuated when cells were also incubated with the antibody ((F3,11) = 4.53; p = 0.033 one-way ANOVA *p < 0.05; control vs LPS + Aβ; ⁺p < 0.05: Newman-Keuls multiple comparison test; LPS + Aβ vs LPS + Aβ+anti-TLR2 antibody). Data are expressed as the percentage of LAMP (a), Arg1 (b) or Iba1 (c) staining in each treatment group vs the control. A total of 10 fields per experiment in triplicate were analysed