Fig. 2

The expression of NLRP3 inflammasomes in BV2 cells or primary microglia, as measured by western blot. a The expression of NLRP3 in BV2 cells at different time points after reoxygenation. b The expression of NLRP3, ASC, pro-caspase-1, and cleaved caspase-1 in BV2 cells among the different groups. c The purity of primary microglia. d The expression of NLRP3, ASC, pro-caspase-1, cleaved caspase-1, pro-IL1β, cleaved IL1β, pro-IL18, and cleaved IL18 in primary microglial cells among the different groups. OGD/R treatment was conducted at 24 h after siRNA transfection. Bar = 100 μm. *p < 0.05, **p < 0.01. OGD/R: oxygen-glucose deprivation/reoxygenation. The OGD continued for 4 h, followed by reoxygenation