Fig. 3

NLRP3 inflammasome pathway expressed in PC12 and bEnd3 cells in the transwell co-culture system after OGD/R was induced by BV2 cells. a The expression levels of NLRP3, ASC, pro-caspase-1, and cleaved caspase-1 in BV2, PC12, and bEnd3 cells in different groups, as measured by western blot. b The changes of NLRP3, ASC, and cleaved caspase-1 in BV2 cells in the transwell co-culture with PC12 cells at different time points after reoxygenation, as measured by western blot. c The changes of NLRP3, ASC, and cleaved caspase-1 in PC12 cells in the transwell co-culture with BV2 cells at different time points after reoxygenation, as measured by western blot. d The changes of NLRP3, ASC, and cleaved caspase-1 in PC12 cells with isolated cultured between the two groups, as measured by western blot. e The changes of NLRP3, ASC, and cleaved caspase-1 in bEnd3 cells among the different groups, as measured by western blot. *p < 0.05, **p < 0.01. OGD/R: oxygen-glucose deprivation/reoxygenation. The OGD continued for 4 h, followed by reoxygenation