Fig. 7

Mitochondrial dysfunction could activate NLRP3 inflammasomes in BV2 cells after OGD/R. a Measurements of the ∆ψm and mtDNA copy numbers in BV2 cells among the different groups. The BV2 cells in OGD/R or OGD/R + diazoxide groups were measured at 24 h after reoxygenation. b The expression levels of NLRP3 and pro-caspase-1 physically associated with ASC in BV2 cells among the different groups, as measured by IP. Anti-ASC antibody was used to immunoprecipitate NLRP3 inflammasome. IB assay for ASC was used as a loading control. c The expression levels of NLRP3, ASC, cleaved caspase-1, IL-1β, and IL-18 in BV2 cells among the different groups, as measured by western blot and ELISA. d The expression of NLRP3, ASC, pro-caspase-1, cleaved caspase-1, pro-IL1β, cleaved IL1β, pro-IL18, and cleaved IL18 in primary microglial cells among the different groups. The 100 μm diazoxide was applied to the cells when got reoxygenation. Bar = 100 μm. *p < 0.05, **p < 0.01. OGD/R: oxygen-glucose deprivation/reoxygenation. The OGD continued for 4 h, followed by reoxygenation