Fig. 8

Mitochondrial protector could rescue the NLRP3 inflammasome pathway expressed in PC12 and bEnd3 cells in the transwell co-cultures after OGD/R. a The expression levels of NLRP3, ASC, pro-caspase-1, and cleaved caspase-1 in PC12 and bEnd3 cells among the different groups, as measured by western blot. b The changes of NLRP3, ASC, and cleaved caspase-1 in PC12 cells among the different groups, as measured by western blot. c The changes of NLRP3, ASC, and cleaved caspase-1 in bEnd3 cells among the different groups, as measured by western blot. d The expression levels of IL-1β and IL-18 in PC12 cells among the different groups, as measured by RT-PCR and ELISA. e The expression levels of IL-1β and IL-18 in bEnd3 cells among the different groups, as measured by RT-PCR and ELISA. The 100 μm diazoxide was applied to the cells when got reoxygenation. *p < 0.05, **p < 0.01. OGD/R: oxygen-glucose deprivation/reoxygenation. The OGD continued for 4 h, followed by reoxygenation